SOP - Silicone Molds using 3D Printed Molds

Standard Operating Procedure

Title: Creation of Silicone Molds Using 3D Printed Molds

SOP Number: SOP-SM-01

Effective Date: In review

Review Date: [Insert Date]

Author: Cassox

Approver: [Approver Name]

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Table of Contents

1. Purpose
2. Scope
3. Responsibilities
4. Definitions
5. Materials and Equipment
6. Safety Precautions
7. Procedure
   • 7.1 Preparation of Work Area
   • 7.2 Preparation of 3D Printed Molds
   • 7.3 Silicone Preparation
   • 7.4 Molding Process
   • 7.5 Curing Process
8. References
9. Appendices

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1. Purpose

This SOP outlines the procedure for creating silicone molds using 3D printed molds, specifically utilizing platinum-cure silicone.

2. Scope

This procedure applies to all personnel involved in the preparation and production of silicone molds in Symbiont Labs.

3. Responsibilities

• Operator: Ensures all steps are followed accurately and safety protocols are maintained.
• Quality Assurance: Verifies the quality of the final silicone molds.

4. Definitions

• Platinum-Cure Silicone: A type of silicone rubber that cures using a platinum-based catalyst, known for its stability and biocompatibility.

5. Materials and Equipment

• 3D printed molds
• Platinum-cure two-part silicone
• Personal protective equipment (PPE): gloves, safety goggles, lab coat
• Isopropyl alcohol (IPA)
• Laminar flow hood
• Vacuum chamber
• Dehydrator
• Mixing containers and utensils
• Top pieces for pressing silicone

6. Safety Precautions

• Wear appropriate PPE at all times to prevent chemical exposure.
• Ensure proper ventilation within the work area.
• Follow manufacturer safety instructions for handling silicone and cleaning agents.

7. Procedure

7.1 Preparation of Work Area

1. Clean the laminar flow hood and surrounding areas thoroughly with isopropyl alcohol (IPA).
2. Activate UV light and flow hood thirty minutes prior to use.
3. Allow the alcohol to evaporate completely before beginning work.

7.2 Preparation of 3D Printed Molds

1. Clean the 3D printed molds using IPA to remove any contaminants and leave exposed to UV light within flow hood. 
2. Allow the molds to dry completely before applying silicone.

7.3 Silicone Preparation

1. Prepare the platinum-cure silicone according to the manufacturer’s instructions, ensuring accurate measurement of parts A and B.
2. Mix the two components thoroughly until a consistent, uniform mixture is achieved. 5-10 minutes. 

7.4 Molding Process

1. Place the silicone mixture in a vacuum chamber and degas it to remove air bubbles.
2. Carefully apply the silicone to the 3D printed mold, ensuring complete coverage.
3. Use a top piece to press the silicone and expel any excess material from the mold.
4. Place the filled molds back into the vacuum chamber for a secondary degassing.

7.5 Curing Process

1.  Transfer the units to a dehydrator set to 104 for curing until fully set.
2. Cure for duration and as advised by manufacturer.

8. References

• Manufacturer’s instructions for platinum-cure silicone
• Safety guidelines for handling chemicals and operating equipment

9. Appendices

• Appendix A: Safety Data Sheets (SDS) for materials used
• Appendix B: Emergency Protocols and Contacts